Isolation of chicken anaemia virus in cell cultures and detection of viral DNA by polymerase chain reaction in naturally infected chicken in Malaysia

Lim, S. S.; Cheah, N. Y.; Tan, C. L.; Azizah Darus; Kono, Y.; Lim, K.T.; Ku, B. D.

Isolation of chicken anaemia virus in cell cultures and detection of viral DNA by polymerase chain reaction in naturally infected chicken in Malaysia

2000

Chicken anaemia virus is known to cause severe anaemia in newly hatched chicks. Although serological reactors to chicken anaemia infection have been detected in the country, the virus has never been isolated from chickens. This paper reports the first isolation of the virus in Malaysia and highlights the application of polymerase chain reaction (PCR) technique for rapid detection of the virus from field specimens.

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