Genetic analysis of Aedes aegypti (L.) in Manila, Philippines: dispersal pattern, and gene expression under Wolbachia infection
2019
Regilme, M.A. | Inukai, T. | Viacrusis, K. | Amalin, D.M. | Watanabe, K.
Anthropogenic land use influences the proliferation patterns of mosquitoes across urban competence. The effect of España Boulevard [Manila, Philippines] in dispersion patterns of dengue of dengue vector was patterns of dengue vector was examined from August through September 2017. A. aegypti were collected in the north and south sides of the road. DNA and RNA are isolated from individual mosquitoes using Qiagen AllPrep DNA/RNA isolation kit. The effect of España Boulevard in dispersion patterns of dengue vector was examined from August through September 2017. A. aegypti were collected in the north and south sides of the road. DNA and RNA are isolated from individual mosquitoes using Qiagen AllPrep DNA/RNA isolation kit. The effect of the road in the population structure was determined using 12 microsatellite loci for molecular analysis. Double digest restriction-site associated DNA sequencing revealed genome-wide single nucleotide polymorphisms. Wolbachia infections were detected vy PCR in seven adult mosquitoes. RNA sequencing explained the difference in the gene expression levels of Wolbachia infected and non-infected adult mosquitoes. Important genes in Wolbachia infected mosquitoes responsible for dengue virus inhibiting properties were analyzed. Control zones along landscapes features in urban areas that can act as barrier or corridor in mosquito dispersal could be determined. If major roads are revealed as barriers, road blocking can be used as as efficient and appropriate spatial unit in studying and controlling the vector.
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