Cry1Ab-resistance detection in the Asian corn borer, Ostrinia furnacalis (Guenee), using diagnostic dose assay and F2 screen
2019
Saballo, K. | Alcantara, E.P.
A two-step detection method using artificial diet overlay bioassay with diagnostic dose of Cry1Ab protein followed by F2 screen of progenies from sib-mated male and female adults that survived the treatment from diagnostics dose assays was utilized for attempting to isolate major non-recessive resistance alleles from the Asian corn borer, Ostrinia furnacalis (Guenee) populations. The number of parental pair-mated male and female adults collected from three sites in Cauayan, Isabela [Philippines] ranged from 123-202 pairs. The total number of families surviving into the F2 generations ranged from 3.9. There were no surviving larval progenies from each F2 family line treated with Bt corn leaf discs. No major non-recessive resistance allele is present in the insect samples collected from the three sites in Isabela.
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