Rice dwarfs virus (RDV) detection in the Nephotettix nigropictus (Stal) and rice plant, and field reaction of rice cultivars against RDV in the Philippines
2019
Rillon, J.P. | Duque, M.J.C. | Alili, R.P. | Imbat, J.B. | Cantila, I,M.B. | Rillon, G.S. | Padolina, T.F. | Tiongco, E.R.
The rice dwarf disease generally occurs in temperate rice growing countries. Its occurrence in the Philippines was reported in 1994 based on transmission studies, electron microscopy of the virus particles, and serological assay of infected plants. A simple nucleic acid amplification technique called the loop-mediated isothermal amplification (LAMP) was applied to detect Rice Dwarf virus (RDV) in field collected insect vector, Nephotettix nigropictus (Stal), individuals and rice plants. Samples were crudely lysated with alkaline lysis method. LAMP successfully detected RDV in both insect vector and plant hosts at 60 deg C in 60 min. Visual scores of rice dwarf diseased plants in the National Cooperative Trial (NCT) 2014 dry season test showed that rice lines developed for the upland ecosystem obtained the highest infection rate (13.89%); nearly as high as the TN1 check plants (15.21%) while the Multi-Adaptation Trial (MAT) lines obtained the lowest (059%). In the 2015 wet season NCT trial, the Traditional rice group recorded the highest percentage of infection (4.81%) followed by those in the direct wet seeded rice (DWSR) category (3.47%) while rice entries in MAT and Transplanted categories obtained the two lowest infection of 1.20% and 1.25%, respectively. The TN1 check variety recorded 2.54 % infection. A 0.4% rice dwarf disease incidence was recorded in a field survey in the rice production area of the PhilRice Midsayap Branch station during the 2015 DS cropping. The average number of N. Nigropictus in the NCT and rice production fields ranged from 0-4.33 for 10 insect net sweeps.
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