Morphological characterization and SSR-based DNA fingerprinting of cassava (Manihot esculenta Crantz) varieties released by the National Seed Industry Council (NSIC)
2019
Vinarao, G.B. | Mendoza, M.R.R. | Villa, N.O. | Dela Viña, C.B. | Abustan, M.A.M. | Lalusin, A.G.
The genetic variability of 47 cassava varieties released by the National Seed Industry Council (NSIC) was evaluated through morphological characterization and DNA fingerprinting using simple sequence repeat (SSR) markers. Phenotypic similarities based on 34 morphological descriptors were observed in varieties sharing a common parent. Fifty (50) polymorphic SSR markers were used to construct DNA fingerprints. A total of 648 polymorphic alleles and 764 unique banding patterns were observed among the varieties. The polymorphism information content (PIC) values ranged from 0.46 to 0.95, with an average of 0.83. Each variety had a unique banding profile indicating that SSR markers were useful distinguishing cassava varieties. A wider genetic diversity of cassava varieties was detected in SSR data (70%) compared with morphological data (50%) showing that SSR markers are more effective in determining the extent of variation between genotypes. The DNA fingerprints of cassava varieties have been successfully generated which can be used as a benchmark for identification and authentication of released cassava varieties.
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