Synergistic Effects of Dietary Vitamin C and Selenium on Induced Methylmercury Toxicity in Juvenile Olive Flounder Paralichthys olivaceus
2017
Lee, Seunghan | Park, Gunhyun | Moniruzzaman, Mohammad | Bae, Jinho | Song, Yujin | Kim, Woong | Bai, Sungchul C
The synergistic effects of dietary vitamin C and selenium (Se) on induced methylmercury (M eHg) toxicity in juvenile olive flounder Paralichthys olivaceus were examined in this study . Nine diets containing 3 different vitamin C levels (0, 200 and 400 mg kg-1 diet in the form of L- ascorbyl-2-monophosphate), 3 different Se levels (0, 2 and 4 mg kg-1 diet in the form of selenomethionine) at a constant level of M eHg (20 mg kg-1 diet in the form of M eHg) were formulated and fed to triplicate groups of juvenile olive flounder with mean weight of 2.00±0.04 g (mean± SD) in semi recirculation system using 32 factorial design. Growth performance and tissue Hg burden were determined after 8 weeks of feeding. Fish fed diets containing 400 mg kg-1 vitamin C together with 2 and 4 mg kg-1 Se (C400Se2 and C400Se4) showed significantly (P<0.05) higher weight gain (WG), specific growth rate (SGR) and feed efficiency (FE). Whereas fish which were under the C400Se4 diet exhibited significantly (P<0.05) higher and protein efficiency ratio (PER) than other feeding groups. Tissue Hg burden in muscle, liver and kidney showed a tendency of increasing with decreasing the levels of vitamin C and Se. However, significantly low tissue Hg burden was observed from fish fed diets containing 400 mg kg-1 vitamin C together with 2 and 4 mg kg-1 Se (C400Se2 and C400Se4). The results suggested that tissue Hg burden could be reduced and M eHg mediated growth problems could be ameliorated by supplementingdietary vitamin C and Se in juvenile olive flounder.
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