Mini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium.
2019
OLIVEIRA, C. C. S. | SOUZA-FABJAN, J. M. G. | FONSECA, J. F. da | BATISTA, R. I. T. P. | BALARO, M. F. A. | SARAIVA, H. F. R. de A. | ALFRADIQUE, V. A. P. | CÔRTES, L. R. | BRANDÃO, F. Z.
葡萄牙语. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017.
显示更多 [+] 显示较少 [-]英语. Sperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen congelado/descongelado de ruminantes domésticos dispensa o uso da heparina em meio capacitante].
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