Poisson distribution-based conventional PCR protocol for quantification of pathogenic fungi in maize
2021
Grăjdieru, Cristina
Currents study aims to optimize and apply a conventional PCR protocol based on digital assay principle for quantification of pathogenic fungi in maize. Conventional PCR based on Poisson distribution is an effective and technically simple method for quantitative analysis of plant pathogens. It doesn’t require specific reagents or sophisticated laboratory equipment. Unlike qPCR, no standard curve is needed for quantifying the number of copies per sample. It permitted to quantify several toxigenic and non-toxigenic fungal pathogens in maize plants and reveal genome impact on the quantity of fungal DNA in host organism. Overall, fungal quantity was lowest in ‘MK01’ and ‘KU123’ and highest in ‘CP137’. High quantity of fungal DNA was also computed for ‘CP148’. For ‘B73’ the fungal DNA quantity was in between the same values for ‘KU123’ and ‘CP148’.
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