Effects of Rhizobium and Arbuscular Mycorrhiza applications on seed yield and some agronomical characteristics of common bean (Phaseolus vulgaris L.)

This study was conducted to reveal the effects of alone and dual inoculation withRhizobium and mycorrhiza under fertilized and non-fertilized conditions on plantgrowth, seed yield and some agronomical characteristics of common bean cv.Zülbiye at the Çarsamba Ambarköprü Research Station of Samsun Black SeaAgricultural Research Institute, Turkey in 2012 and 2013. The experiments were laidout in a split-split plot design according to a randomized complete block design(RCBD) with four replications. Fertilizer treatments (G0: non-fertilized and G1:fertilized at the rate of 20 kg N/da ve 60 kg P2O5/da), Rhizobium inoculationtreatments (R0: non-inoculated with Rhizobium, R1: inoculation with standardinokulum for bean, R2: inoculation with the liquid mixture of local Rhizobiumisolates from bean cultivated fields of Samsun province), mycorrhiza inoculationtreatment (M0: non-inoculated with mycorrhiza and M1: inoculation withmycorrhiza) were placed to main, su and sub-sub plots, respectively. In 2012,fertilization treatment decreased nodule number (47.50 nodules/plant) whencompared with non-fertilized control teratment (57.15 nodules/plant). Inoculation ofbean seeds with Rhizobium (R1 and R2) significantly increased the nodule numberper plant when compared with non-inoculated control treatment in both 2012 andcombined analyses, but a significant difference was not found between commonlyused standard inoculant (R1) and inoculant mixture (R2) consisted of localRhizobium isolates with respect to number of nodule formed in the roots. In 2012and combined analyses, the number of nodules per plant was significantly lower inmycorrhiza inoculation treatment (46.16 and 52.31 nodules/plant, respectively) thanthat in non-inoculated treatment (58.49 ve 58.39 nodules/plant, respectively). Thiscan be attributed to decreasing number of nodule formed by the Rhizobium due tocompetition between Rhizobium and mycorrhiza. The effects of fertilizer,Rhizobium and mycorrhiza treatments on plant height, number of seeds per plant,seed yield per plant and per hectare, 100 seed weight, seed protein content, seedphosphorus content, root length, root fresh and root dry weight were found nonsignificantin 2012, 2013 and combined analyses. Mycorrhizal inoculation highlysignificantly increased mycorrhizal colonization rate (73.17%) when compared tonon-inoculated plants (40.50%). The effect of mycorrhizal inoculation on P, Ca, Mg,Fe, Zn, Mn, Cu and Na contents of seeds was not significant.January 2017, 157 pages