Cytopathic effect of ovine adenovirus type I in the primary cell-cultures of ovine and caprine origin [in sheep and goats]
1986
Dubey, S.C. | Kumar, N. | Sharma, S.N. (Punjab Agricultural Univ., Ludhiana (India). Coll. of Veterinary Science)
Two local isolates of ovine adenovirus I (DI6 and DI6F3) were recovered from foetal lamb kidney cells and propagated in lung cells and lamb testis cells. Similar cell cultures of goat origin also supported the multiplication of these isolates producing characteristic cytopathic effects. The kidney cell-culture showed high titre followed by testis and lung cell-cultures. Cytopathic effects appeared later in lung cells than in kidney and testis cell-cultures. Indirect immunofluorescent test revealed characteristic intranuclear fluorescing viral inclusions in homologous and heterologous cell-cultures
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