Preservation methods of Xanthomonas campestris pv. oryzae in relation to virulence and colony type variation
1986
Hwang, I.G. | Cho, Y.S. (Seoul National Univ., Suwon (Korea R.). Dept. of Agricultural Biology)
Effects of preservation methods on viability and virulence of Xanthomonas campestris pv. oryzae were investigated. The incidence of colony-type variants from freeze-dried and deep-frozen cultures was also determined. The suspending medium for freeze-dried cultures containing 10 % sucrose and 1 % gelatin showed the highest viability, and the virulence was well maintained in the suspending medium containing 2 % dextrin, 0.5 % ascorbic acid, 0.5 % ammonium chloride, 0.5 % thiourea, and 0.85 % NaCl. Serially transferred cultures became attenuated. Rough colonies which had wrinkled surface occurred at a frequency of 1.9 % to 15.8 % during freeze-drying and freezing
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