Intergeneric transfer of nitrogen fixation genes from Rhizobium leguminosarum by RP4 :: Mu cts
1986
Huh, Y.J. | Lee, Y.N. (Korea Univ., Seoul (Korea R.). Dept. of Biology)
Nitrogen fixation (nif) genes of Rhizobium leguminosarum were transferred to nif Klebsiella pneumoniae and E. coli by conjugation after partial heat induction of RP4 :: Mu cts in Rhizobium R+ transconjugant, and the hybrid plasmids in the transconjugant strains were isolated and characterized. In order to transfer the nif genes from Rhizobium, the hybrid plasmid RP4 :: Mu cts was transferred by conjugation from E. coli to the symbiotic nitrogen fixer, R. leguminosarum. After stability test, the RP4 :: Mu cts in Rhizobium R+ transconjugant was subjected to partial heat induction by culturing it statically at 38 deg C for 16 hrs, and then conjugated with the nif defective mutant strains of K. pneumoniae or nif mutant strains of E. coli having whole nif gene plasmid
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