Gene-technological strategies for producing virus resistance in plants. Can the problem of sugarbeet rizomania be solved?
1988
Koenig, R. (Biologische Bundesanstalt fuer Land- und Forstwirtschaft, Braunschweig (Germany, F.R.). Inst. fuer Viruskrankheiten der Pflanzen)
The replication of viruses proceeds in a number of steps in which the individual virus-specific products, such as replicase, nucleic acid and coat protein, are synthesized in suitable proportions and in a strict order of sequence. This process is apparently regulated by feed-back mechanisms in which late gene products may control the early steps of replication. With a number of viruses the coat protein blocks either the production of viral replicase or the transcription of the viral nucleic acid. The incorporation of complementary DNAs to viral genes or virus gene-related nucleotide sequences in the host genome and their transcription or expression in the host cells may disturb the regulatory mechanisms of a virus to such an extent that the replication of the virus becomes impossible. In such transgenic plants either viral genes or their recognition sites or virus-encoded enzymes, e.g. the replicase, are blocked. The blockage of genes or their recognition sites can be achieved by virus-related proteins, e.g. the coat protein or mutated replicases can be blocked by defective viral RNA sequences which contain the replicase-binding sites or by satellite RNAs. A group of researchers at the Institutes of Biochemistry and of Plant Virus Research of the Federal Biological Research Center in Braunschweig presently investigate which of these strategies appears to be most suitable for conferring rizomania resistance to sugarbeets
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