Direct introduction of fluorescein isothiocyanate-conjugated albumin into intact macroconidia of Fusarium oxysporum f. sp. lycopersici by electroporation
1989
Matsuda, Y. (Kinki Univ., Higahi-Osaka (Japan). Lab. of Plant Pathology) | Toyoda, H. | Nishiguchi, T. | Ouchi, S.
An electroporation procedure for the introduction of fluorescein isothiocyanate-conjugated albumin into intact macroconidia of Fusarium oxysporum f. sp. lycopersici was performed in this paper. FITC-albumin was used to establish an efficient electroporation procedure because its presence in spores could be easily detected by fluorescence microscopy. The uptake of fluorescein isothiocyanate-conjugated albumin into spores was successfully mediated by high voltage electrical pulses at field strength of 10 KV/cm. In electroporation of 10(6) macroconidia per ml with the present system, 1,2 x 10(5) spores incorporated the protein, and then 1,3 x 10(4) normally germinated, elongated vegetative hyphae, and produced spores
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