Cultivar response and a possible biochemical basis of resistance of soybean seed [Egypt]
1988
Shehata, M.R.A. | Wagih, E.E. | Farag, A.S. | Dawood, M.K.
Aspergillus flavus, A. niger, and A. ochraceous were isolated from naturally infected soybean seeds and proved to be pathogenic when tested in vitro on apparently healthy seeds of the cultivar Williams. Fifteen cultivars were evaluated for resistance to infection by the three species of Aspergillus and found to differ in this respect. Crawford cv. Was found to be most resistant to infection whereas Forrest cv. was the most susceptible. The other thirteen cultivars occupied intermediate positions as they showed similar reaction (60-80% infected seeds) when evaluated by the seed colonization test. No stimulation or inhibition of the in vitro fungal growth was obtained by seed exudates of Forrest or Crawford cultivar, respectively. Also, no change in host response was obtained when seeds of Crawford or Forrest cultivar were treated by the seed exudates of the other one. Upon analysis of soluble protein fraction of Crawford and Forrest seeds, both qualitative and quantitative differences were observed. The same was true as to peroxidase isozyme content of the two cultivars. Three Carwford-specific isozyme with Rf values of 0.09, 0,46-0.50, and 0.57 are suspected of being involved in the high degree of resistance demonstrted by the cultivar Crawford to Aspergillus spp. infection.
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