An improved method of seed stratification in grapevine in the breeding process
1991
Michlovsky, L. (Slechtitelska Stanice Vinarska, Perna u Mikulova (CSFR))
Suitability of different methods of seed stratification in grapevine and of physical and chemical methods of germination increase in the breeding process was tested in 1981-1987. The application of H2O2 and GA-3 did not have any significant effects, moreover, morphological changes were observed in seedlings. Germination is strongly influenced by the year and genetic differences. A simple procedure, suitable for breeding practice, was prepared pursuant to the results of experiments. Dry seeds, harvested from ripe grapes, are kept in winter at a dry place at the temperature of 5-20 degrees C. Before sowing the seeds are soaked in water for 30-40 day; water must be changed at the beginning. For another 15-30 days the seeds were kept in cold at a temperature of 2-5 degrees C; this regime can be combined with alternating temperatures (5-10 days the temperatures of 5 and 20 degrees C for 16 and 8 hours). After the seeds are planted in substrate, alternating temperatures of 20 and 30 degrees C are maintained in the intervals of 16 and 8 hours. After 10-20 days the seeds begin to germinate in a spontaneous and regular manner and high germination is achieved. The results of seed stratification in sand were worse than the outcome of stratification in water and cold; moreover, the former method requires greater labour input.
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