Optical activity of the background caused by sheep organ tissue extracts and its effect on spectrophotometric determination of the herbicide bentazone
1991
Orinak, A. | Holovska, K. | Budinska, V.
Nowadays mainly triazine herbicides are used for treating cultural plant against weeds. Many methods for their determination as well as for their extraction and purification have been developed, but the latter apply mainly for the determination in plant material. In the present publication the effect of the animal organ tissue extracts on the residue determination by optical methods is investigated. The background materials obtained after extraction of sheep muscle, liver, kidney, heart and lung with acetonitrile, acetone and hexane each are investigated by means of an infrared spectrometry. Bentazone was chosen as the model pesticide and had been toxicologically tested with two experimental groups of sheep throughout three months administered with two different dose rates. Since the main chromophore of the bentazone molecule is represented by a ketonic group which is used for the spectrophotometric determination of the substance, the elimination of the background at the respective UV region should be based on suppression of the optic activity of equivalents of similar absorbing chromophores as owned by the bentazone molecule. For this reason the cleaning capacities of the separcol SI C 19, SI C 1 and Carb of Czechoslovak provenience were examined. After elimination of interfering background bentazone determination was verified at a wavelength of 219 nm
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