Testing the efficiency of disinfectants for control of virus - results achieved in disinfection experiments and proposal for proofing guide lines
1992
Paludan, N. (Research Centre for Plant Protection, Lyngby (Denmark). Dept. of Plant Pathology)
To avoid contamination and infection of pathogens, plants have to be grown under high hygienic conditions. In order to achieve this, disinfectants with high efficiency are needed. While lines of direction have been made for proofing disinfectants in ornamentals concerning bacteria and fungus (Anon. 1989) no procedure exists for viruses. To achieve reproducible results experiments concerning the efficiency of different disinfectants for control of viruses were started ar the Research Centre for Plant Protection in Lyngby in 1988 as described in this paper. The following methods were used: - suspension test with short time treatment at a high infection rate to show the ability of disinfectants to virus inactivation - suspension test with long time treatment at a lower infection rate with and without organic substances added to show the efficiency of disinfectants to act as surface disinfectants - knife decontamination test to demonstrate the efficiency of disinfectants to inactivate existing virus on implements. The efficiency of a disinfectant was dependent on the virus species present, the concentration of the disinfectant used and the period of treatment. Increasing the concentration of the disinfectant and prolonging the period of treatment both increased the virus inactivation substantially. Tomato mosaic virus turned out to be one of the virus most difficult to inactivate and was therefore very suitable as a test virus. Addition of peat to the disinfectant did not cause any or only a very slight reduction of the efficiency of the disinfectant. Elimination of virus on implements was achieved after 5 to 10 minutes of treatment, a shorter period of treatment or only dipping did not eliminate the virus
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