Soil-borne viruses of melons and cucumbers in France
1990
Campbell, R.N. (California Univ., Davis (USA). Plant Pathology) | Lecoq, H. | Wipf-Schiebel, C. | Sim, S.T.
Several viruses that produced necrotic lesions on most host range plants were isolated from systemically infected cucurbits in French glasshouses or plastic tunnels between 1979 and 1989. Single-lesion transfers were done for each of nine isolates. The isolates were identified serologically with the protein A-sandwich enzyme-linked immunosorbent assay (PAS-ELISA). Five of the isolates from cucumber and melon were melon necrotic spot virus (MNSV). Two isolates from cucumber were A serotypes of tobacco necrosis virus (TNV). One isolate from cucumber was a D serotype of TNV, and one isolate from cucumber was petunia asteroid mosaic virus (PAMV). The MNSV isolates were restricted to cucurbit hosts and could be divided into strains originating from cucumber or those from melons by the differential reaction of Marketer cucumber. The cucumber strains caused 2-4 mm necrotic lesions on cucumber cv Marketer in 4-5 days whereas the melon strains produced pinpoint necrotic lesions. The present paper is the first report of a D serotype of TNV on cucumber in Europe or of natural infection of cucurbit hosts by PAMV
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