Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from Impatiens sp. in the Netherlands
1992
Avila, A.C. de (Department of Virology, Wageningen (Netherlands)) | Haan, P. de | Kitajima, E.W. | Kormelink, R. | Resende O, R. de | Goldbach, R.W. | Peters, D.
This paper describes the characterization of a distinct tomato spotted wilt virus (TSWV) isolate from New Guinea impatiens (Impatiens sp.) in the Netherlands. Several plant species responded in a hypersensitive way to this isolate, denoted NL-07, but the symptoms on Impatiens do not differ from those caused by other TSWV isolates. Except that the S RNA is slightly smaller, the protein and RNA composition is identical to that established for the isolates described. Comparative studies using ELISA, Western blotting and immunogold labelling techniques clearly demonstrate that NL-07 has a distinct nucleocapsid protein from BR-01 (common type TSWV), BR-03 and SA-05 and moreover it is very closely related to TSWV-1. Northern blot analysis, supporting the serological data, revealed a lack of significant nucleotide sequence homology between BR-01, NL-07 and TSWV-1. Cytopathological studies of NL-07 displayed clusters of virus particles in the cisternae of the endoplasmatic reticulum together with paracrystalline arrays of rigid rod-like fibers, formed by the non-structural protein. Our results show that TSWV classification based on ELISA using monoclonal and polyclonal antibodies against the nucleocapsid protein is stable and safe, even when deletions in L RNA segment occur or when the virus becomes morphologically defective. We propose to name this virus Impatiens necrotic spot virus
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