Effect of UV-C on peroxidase isoenzymes in axillary bud cultures of Vitis species differing in fungal resistance to Plasmopara viticola
1994
Zapata, J.M. | Calderon, A.A. | Pedreno, M.A. | Munoz, R. | Ros Barcelo, A. (Univ. of Murcia (Spain). Dept. of Plant Biology)
The effect of shortwave (250 nm) UV radiation (UV-C) on the level of peroxidase activity and peroxidase isoenzyme patterns in leaves of resistant ([Vitis vinifera x Vitis riparia] x Vitis rupestris and Vitis rupestris) and susceptible (Vitis vinifera) grapevine species to Plasmopara viticola (downy mildew) was studied. The results show that although UV-C did not produce significant changes in peroxidase activity in susceptible species, and only minor changes in resistant species, treatment with UV-light induces an acidic isoperoxidase (isoperoxidase A1), capable of oxidising 4-hydroxystilbenes in resistant species. It was named HSPrx 2. Since peroxidase is apparently the enzyme responsible for eta-viniferin synthesis from resveratrol in grapevines, a close relationship between this peroxidase isoenzyme and eta-viniferin synthesis which occurs in grapevine leaves after UV-C treatment must be expected.
显示更多 [+] 显示较少 [-]