Chromosome mapping in Alcaligenes eutrophus CH34
1993
Sadouk, A. (Centre d'Etudes de l'Energie Nucleaire and Vlaamse Instelling voor Technologisch Onderzoek, Mol (Belgium). Lab. de Genetique et Biotechnologie) | Mergeay, M.
Mutants and mobilizing plasmids were developed as genetic tools in Alcaligenes eutrophus CH34. In order to map the chromosome, spontaneous and ethyl methane sulphonate (EMS)-induced mutants (mostly auxotrophs) were isolated. Another source of mutants was provided by the phenomenon of temperature-induced mortality and mutagenesis that is observed at 37 degrees C and is characteristic of many metallotolerant strains of A. eutrophus. Plasmid pULB113 (RP4: miniMu) was used to map the available mutations. 25 loci were ordered in a circular map. pMOL50, a rearranged derivative of plasmid pMOL28, which was obtained in a survivor at 37 degrees C and displayed chromosome mobilizing activity (Cma(+)), was also used to mobilize chromosomal markers: resulting linkages were stronger than with pULB113, allowing confirmation of the circularity of the A. eutrophus CH34 chromosome with a small number of crosses.
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