Cytological and molecular characterization of a chromosome interchange and addition lines in Cadet involving chromosome 5B of wheat and 6Ag of Lophopyrum ponticum
1993
Kim, N.S. (Canada Agriculture, Ottawa (Canada). Research Branch. Plant Research Centre) | Armstrong, K.C. | Fedak, G. | Fominaya, A. | Whelan, E.W.P.
Efforts to transfer wheat curl mite (Eriophyes tulipae Keifer) resistance from Lophopyrum ponticum 10X (Podb.) Love to bread wheat (Triticum aestivum L.) have resulted in the production of a number of cytogenetic stocks, including an addition line of 6Ag, a "ditelo" addition line, and a wheat-Lophopyrum translocation line. Characterization of these lines with C-banding, in situ hybridization with a Lophopyrum species-specific repetitive DNA probe (pLeUCD2), and Southern blotting with pLeUCD2 and a 5S ribosomal DNA probe (pScT7) confirmed that the distal portion of the short arm of 6Ag was translocated onto the distal portion of 5BS (5BL.5BS-6AgS). It was also determined that the "ditelo" addition was an acrocentric chromosome of 6AgS.
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