Characterization of Thinopyrum bessarabicum chromosome segments in wheat using random amplified polymorphic DNAs (RAPDs) and genomic in situ hybridization
1993
King, I.P. (John Innes Centre, Norwich (United Kingdom). Cambridge Lab.) | Purdie, K.A. | Rezanoor, H.N. | Koebner, R.M.D. | Miller, T.E. | Reader, S.M. | Nicholson, P.
Ten random amplified polymorphic DNA (RAPD) markers specific to chromosome 5E(b) of Thinopyrum bessarabicum were detected. Genomic in situ hybridization and standard cytological observations revealed that 6 of the markers are located on the 5E(b) short arm and four are located on the 5E(b) long arm. These RAPD markers have been used to confirm the identity of putative 5E(b) (5A) and 5E(b) (5D) substitution individuals. The potential of RAPDs for the detection of wheat/alien recombinants is discussed.
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