National survey of viruses in pastures of subterranean clover, 2. Statistical methodology for large scale quantitative ELISA [Trifolium subterraneum; Australia]
1993
Muller, W.J. (Commonwealth Scientific and Industrial Research Organisation, Canberra (Australia). Inst. of Natural Resources and Environment) | Helms, K. | Waterhouse, P.M. (Commonwealth Scientific and Industrial Research Organisation, Canberra (Australia). Div. of Plant Industry)
Statistical methodology was applied to a survey of time-course incidence of 4 viruses (alfalfa mosaic virus, clover yellow vein virus, subterranean clover mottle virus and subterranean clover red leaf virus) in improved pastures in southern regions of Australia, with samplings in each winter and spring over 3 years. The 100 samples per paddock collected at each time of sampling provided detection probabilities of 0.63 and 0.87 for 1 percent and 2 percent infection respectively. A microtitre plate design for ELISA was developed to include 60 field samples, 10 glasshouse-grown healthy control samples and 6 glasshouse-grown samples infected with the virus under examination. This design was used on 806 plates for each of the 4 viruses tested. A generalized linear model with binomial errors and logit link was used for adjusting the virus incidences for biases due to paddocks and-or districts not sampled on some occasions. The same model also proved to be the most appropriate for investigating the effects of year, season and district on virus incidence.
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