Successful fertility experiments with cryopreserved spermatozoa of barramundi, Lates calcarifer (Bloch), using dimethylsulfoxide and glycerol as cryoprotectants
1993
Palmer, P.J. (Queensland Dept. of Primary Industries, Deception Bay (Australia). Southern Fisheries Centre) | Blackshaw, A.W. (Queensland Univ., St Lucia (Australia). Dept. of Physiology and Pharmacology) | Garrett, R.N. (Queensland Dept. of Primary Industries, Cairns (Australia). Northern Fisheries Centre)
Semen diluted (1:4 v-v) and frozen (minus 196 deg. C) with 5 percent dimethylsulfoxide (DMSO) or 10 percent glycerol (final concentration) as cryoprotectants was used to inseminate freshly stripped ova. When eggs and sperm were mixed before the addition of seawater, semen frozen with DMSO as cryoprotectant gave a mean hatch rate (84.1 percent) no different from that of unfrozen semen diluted with Ringer's solution (80.7 percent) or with DMSO (83.7 percent), but higher than that of semen frozen with glycerol (60.9 percent). Adding sperm to seawater 30 s before mixing with eggs did not improve the fertility of sperm cryopreserved with glycerol. Eggs inseminated with glycerol-cryoprotected sperm showed higher mortality during incubation than those inseminated with DMSO-cryoprotected sperm. Sperm held in liquid nitrogen for 90 days with DMSO as cryoprotectant yielded acceptable fertilization and hatching rates with semen-to-ova ratios of up to 1:100 (v-v), and produced fish with no apparent abnormalities over a 29-day period after hatch. These results show that cryopreservation of L. calcarifer sperm is feasible and well suited to a variety of hatchery purposes.
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