DNA restriction fragment length analysis and DNA fingerprinting of the European barley powdery mildew population (Erysiphe graminis hordei)
1992
Koch, G. (Giessen Univ. (Germany). Inst. fuer Pflanzenbau und Pflanzenzuechtung 2. Biometrie und Populationsgenetik) | Koehler, W.
Selection of resistant Hordeum vulgare varieties and the application of fungicides are effective on the adaptation of Erysiphe graminis populations. This was analysed using RFLP and DNA fingerprinting. Looking for similarities between individual isolates one large cluster was found which consists of identical fingerprint patterns. Most of these isolates derived from the same sampling route expressed the same MLH and the same pathotype, indicating a high probability that these isolates originated from one clone. For the total population a high level of individual variation (fingerprints) with clear dissimilarities between Denmark/England in the north, Italy in the south and the other countries were found. It is supposed that the northwestern European population consists of not clearly separated local subpopulations which intermix and does not evolve independently. If it is assumed that virulence and fungicide selection is low or not present in North-ltaly, then the higher variance between the Italian subsamples, left demonstrates the natural occurring variability in the pathogen population. The opposite was found for the German (Flensburg) subsample. Under the regime of virulence and fungicide selection, genetic variation can only be maintained for those traits which are significantly variable like fingerprints and not for most of the gene markers.
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