Establishment and characteristics of a cell line from fetal bovine thyroid (FBTY)
1994
Fischer, O. | Pecuchova, L. | Zendulkova, D. | Horinova, Z. (Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)) | Machatkova, M.
Characteristics of a cell line from fetal bovine thyroid were tested in the 150th passage. The line was adapted to growth in the minimal essential medium supplemented with 10 % bovine fetal serum and it had an epithelioid morphology. After seeding of 10,000 cells/ml, the culture density was higher than 1,000,000 cells/ml on day 6. The FBTY line was heteroploid. The average number of chromosomes was 48.8 +/- 10.0 and only 1 % of mitoses had a diploid number of chromosomes (2n=60). Species specificity (Bos taurus) was confirmed by indirect immunofluorescence. Besides pronounced fluorescence with rabbit antisera against bovine proteins, weak reactions with antisera against ovine, caprine and swine proteins were observed. The FBTY line was susceptible to two strains of parainfluenza 3 virus, six strains of infectious bovine rhinotracheitis virus and bovine adenoviruses of the serovars 1, 2, 3, and 8.
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