Variants within the 5'-flanking regions of bovine milk protein genes: 1. K-casein encoding gene
1994
Schild, T.A. (Hohenheim Univ., Stuttgart (Germany). Fachgebiet Tierzuechtung. Inst. fuer Tierhaltung und Tierzuechtung) | Wagner, V.A. | Geldermann, H.
In order to identify DNA variants within the 5'-flanking region of the bovine K-casein (KCn)-encoding gene, this area of the gene from 13 cows belonging to seven breeds (Holstein Friesian, Brown Swiss, German Simmental, Jersey, Galloway, Scottish Highland and Ceylon Dwarf Zebu) was analysed. For each individual, about 1 kb of the 5'-flanking region including exon 1 was amplified by polymerase chain reaction (PCR). The biotinylated PCR product was immobilized on magnetic beads followed by direct bidirectional sequencing using an automated DNA sequencer. 15 DNA variants were identified, some of which are located within potential regulatory sites and possibly involved in the expression of the K-casein encoding gene.
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