Development and evaluation of a laboratory-based technique for screening somaclonal regenerants of watercress (Rorippa nasturtium-aquaticum) for resistance to crook root disease (Spongospora subterranea f. sp. nasturtii)
1994
Arnold, D.L. (Bath Univ. (United Kingdom). School of Biological Sciences) | Flegmann, A.W. | Clarkson, J.M.
A laboratory based method has been developed for infecting watercress with the crook root fungus (Spongospora subterranea f. sp. nasturtii). This utilised plastic trays of nutrient solution on which watercress shoots, supported by floats, were grown for a pre-inoculation period of 14 days before an inoculum consisting of 1 g of crook roots was added. Seven watercress somaclones derived from plantlets regenerated from callus of a parental commercial control line (clone A) were tested on three separate occasions. No variation within the parental control plants was seen but significant variation both between and within the individual somaclones over successive tests was observed. Two somaclones showed increased resistance to crook root disease compared with parental control plants
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