Cloning and analysis of a FLO5 flocculation gene from S. cerevisiae
1994
Bidard, F. (Institut National de Recherches Agronomiques, Montpellier (France). Inst. des Produits de la Vigne. Lab. de Microbiologie et Technologie des Fermentations) | Blondin, B. | Dequin, S. | Vezinhet, F. | Barre, P.
A yeast flocculation gene was isolated from a genomic library of an FLO5 strain of Saccharomyces cerevisiae on the basis of its ability to trigger flocculation in a non-flocculent strain. Characterization of the cloned gene by restriction mapping, Southern analysis, and chromosome mapping have shown that it corresponds to a FLO5 gene previously located on chromosome 1 and that this gene is related to the already described FLO1 gene. A study of gene expression in different yeast strains has indicated that, while this gene is dominant, its expression can be suppressed in some genetic backgrounds. A Northernblot analysis has demonstrated that the same 5000-nt transcript was present in an FLO5 and an FLO1 strain. A gene disruption experiment has led to the conclusion that another flocculation gene is present and can be active in the FLO5 strain used.
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