Detection of carbaryl residues in vegetables from certain areas in Selangor and Cameron Highlands [Malaysia] by ELISA [Enzyme-linked Immunosorbent Assay]
1993
Zauwiyah, I. | Azimahtol Hawariah, L.P. (Kebangsaan Malaysia Univ., Bangi (Malaysia). Jabatan Biokimia) | Maimon, A.
A highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) is developed for the detection of carbaryl. Polyclonal antibodies with a hapten carbaryl-bovine serum albumin by immunization with a hapten carbaryl-bovine serum albumin (BSA) conjugate. Antibodies with the highest titer were used to obtain a standard curve for the quantification of carbaryl in vegetable samples obtained from certain areas of Selangor (Kepong, Ulu Yam and Tanjung Karang) and Cameron Highland. As comparison, the carbaryl residues in the vegetable samples were also detected using HPLC (High Pressure Liquid Chromatography)
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