Control of replication of the Lactobacillus pentosus plasmid p353-2: evidence for a mechanism involving transcriptional attenuation of the gene coding for the replication protein
1994
Pouwels, P.H. (TNO Medical Biological Lab., Rijswijk (Netherlands). Dept. of Molecular Genetics and Gene-technology) | Luijk, N. van | Leer, R.J. | Posno, M.
The synthesis of plasmid DNA and of RNA encoded by the replication protein gene (rep) of plasmid p 353-2 of Lactobacillus pentosus was studied for the wildtype plasmid and for a mutant plasmid with a deletion in the 5' untranslated region of the rep gene. Plasmid p353-2 codes for two countertranscript RNAs (CT-RNA) of approximately 75 and 250 nucleotides transcribed from the 5' untranslated region of the rep gene, in opposite directions. In a mutant plasmid with a deletion of the promoter and part of the CT-RNA-encoding sequence which shows a 5- to 10-fold increase in copy number compared to the wild-type plasmid, no CT-RNA could be detected. In the wild-type plasmid more than 90% of transcription initiated at a promoter upstream of the rep gene is prematurely terminated to form a 190 nucleotide truncated RNA, whereas in the mutant plasmid nearly all transcripts reach a size (1100 nucleotides) corresponding to that of the rep gene. A model is presented for the role of CT-RNA in control of plasmid replication, similar to that previously presented for the staphylococcal plasmid pT181, involving a mechanism of transcriptional attenuation of rep RNA at a site just upstream of the rep gene.
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