Genetic studies on poplar clones: 3. Investigation of mitochondrial DNA
1989
Radetzky, R. (Goettingen Univ. (Germany). Abteilung fuer Forstgenetik und Forstpflanzenzuechtung)
Restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA) can be used as a measure of diversity and relatedness between species and subspecies in a large number of economically important plant genera. Because the higher plant mitochondrial genome is neither as immensely large and complex as the nuclear genome nor relatively simple or small as the chloroplast genome, it is readily analyzable subsequent to restriction endonuclease digestion, electrophoresis, and staining with ethidium bromide. Intact mitochondria from callus tissue of different poplar clones were purified by isopycnic centrifugation in Percoll. Isolated mtDNA was subsequently digested with hexanucleotide restriction enzymes to detect variations between poplar clones; for instance BamH 1 digestions led to different restriction patterns, which differ in a few number of fragments among the different poplar clones studied. An alternative procedure for detection of mtDNA fragments after digestion of mtDNA or total DNA (leaf tissue) by application of specific "non-radioactively labeled" mtDNA probes (Southern-hybridization) is in progress.
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