The influence of anticoagulant, storage temperature and time on equine plasma lactate concentration [horses]. [Short contribution]
1994
King, C.M. | Rose, R.J. (Sydney Univ. (Australia). Dept. of Veterinary Clinical Sciences) | Evans, D.L. (Sydney Univ. (Australia). Dept. of Animal Science)
A variety of conditions were examined to reflect field conditions under which blood samples from horses are handled and stored in Australia for subsequent lactate analysis. It was found that, for a range of plasma lactate concentrations below 20 mmol per L, lithium heparin may be used as an anticoagulant provided blood samples are kept below 4 deg. C., and analysed within 96 h of collection. If fluoride-oxalate is used as an anticoagulant, blood samples may be kept at temperatures as high as 31 deg. C. for as long as 96 h, without significant change in plasma lactate. For higher concentrations of plasma lactate, lithium heparin is an acceptable anticoagulant, provided that blood samples are stored in a refrigerator and analysed (or the plasma separated) within 24 h of collection.
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