The heat-shock response and expression of heat-shock proteins in wheat under diurnal heat stress and field conditions. [Workshop paper]
1994
Nguyen, H.T. | Joshi, C.P. | Klueva, N. | Weng, J. | Hendershot, K.L. (Texas Tech Univ., Lubbock (USA). Dept. of Plant and Soil Science and Inst. for Biotechnology) | Blum, A. (Volcani Centre, Bet Dagan (Israel). Inst. of Field Crops)
In a field experiment, flag leaf blade temperatures were obtained and leaf blades collected for northern blot analysis using HSP 16.9 cDNA as a probe. Temperatures of leaf blades ranged from 32 to 35 deg. C. Messenger RNAs encoding a major class of low molecular weight heat shock proteins (HSPs), HSP 16.9, were detected in all wheat genotypes examined. The results suggested that HSPs are synthesised in response to heat stress under agricultural production, and furthermore, that HSPs are produced in wheats differing in geographic background. In the controlled growth chamber experiment, HSP expression in 2 wheat cultivars, Mustang (heat tolerant) and Sturdy (heat susceptible) were analysed to determine if wheat genotypes differing in heat tolerance differ in in vitro HSP synthesis (translatable HSP mRNAs) under a chronic, diurnal heat-stress regime. Leaf tissues were collected from seedlings over a time-course and poly (A) plus RNAs were isolated for in vitro translation and 2-D gel electrophoresis. The protein profiles shown in the 2-D gel analysis revealed quantitative differences of individual HSPs between these 2 wheat lines and some unique HSPs which were only found in the heat tolerant line. There was evidence of a correlation between HSP synthesis and heat tolerance in wheat under a simulated field environment. Further genetic analysis of HSPs in a segregating population is warranted.
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