Interior spruce seedlings compared with emblings produced from somatic embryogenesis. II. Stock quality assessment prior to field planting
1994
Grossnickle, S.C. (British Columbia Research Incorporated, Vancouver, BC, Canada.) | Major, J.E.
Interior spruce (Picea glauca (Moench) Voss X Picea engelmannii Parry) seedlings and emblings produced through somatic embryogenesis tissue culture were removed from frozen storage and tested with a stock quality assessment procedure prior to spring planting. Seedlings, compared with emblings, had greater height as well as shoot and root dry weights. Seedlings and emblings had similar root-collar diameter, buds per total shoot length, and needle primordia in their terminal buds. Seedlings and emblings had similar shoot form (i.e., branch quotient) and balance between their shoot and root system (i.e.. plant water balance ratio). Emblings, compared with seedlings, had a lower osmotic potential at turgor loss point and symplastic fraction. plus a higher dry weight fraction and total turgor. Seedlings and emblings had similar osmotic potential at saturation and relative water content at turgor loss point. Seedlings and emblings had similar resistance to water movement through the plant-atmosphere continuum (Rpac) at 22 degrees C root temperature, though emblings had greater Rpac at 7.5 degrees C root temperature. Seedlings had greater net photosynthesis (Pn) than emblings over a 14-day period at both 7.5 and 22 degrees C root temperatures. Seedlings, compared with emblings, had greater root development over a 14-day period at 22 degrees C root temperature, though they had low and similar root development at 7.5 degrees C root temperature. Both Pn and needle conductance (gwv) decreased as predawn shoot water potential (psi pd) declined in seedlings and emblings. Nearly complete stomatal closure occurred at psi pd less than -1.5 MPa, while similarly low Pn readings (seedlings: -0.01 +/- 0.04 micromole m-2. s-1; emblings: 0.02 + 0.04 micromole m-2. s-1) occurred between a psi pd of -1.0 and -2.0 MPa
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