Rapid cryopreservation of sheep embryos by direct transfer into liquid nitrogen vapours at minus 180 deg. C

Szell, A.; Zhang, J.; Hudson, R.

Rapid cryopreservation of sheep embryos by direct transfer into liquid nitrogen vapours at minus 180 deg. C

1990

Szell, A. | Zhang, J. | Hudson, R. (Western Australian Dept. of Agriculture, Katanning. Great Southern Agricultural Research Inst.)

It is demonstrated that rapid cryopreservation is feasible with sheep embryos; a survival rate of 32 percent was achieved with blastocysts and late blastocysts that were exposed to the vitrification medium for 0.5 min at 4-12 deg. C. Longer exposures and earlier development stages reduced the success of the technique.

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