Fluorescence in situ hybridization - a tool for detecting interspecies hybrids in plants
1995
Sinkovic, T. | Batic, F. | Javornik, B. (Ljubljana Univ. (Slovenia). Biotechnical Fac., Agronomy Dept.)
Preliminary results and methodology related to the use of the fluorescence in situ hybridization technique for detection of plants hybrids are presented. Total genomic DNA probe preparation and the non-isotopic biotin probe labelling technique is presented. The use of an excess of unlabelled competitor DNA from a related plant species gives more constant results in determining whole plant genomes with in situ experiments on plant hybrids. Preliminary results of flourescence in situ hybridization for detecting alien rye chromatin and localisation on metaphase chromosomes and interphase nuclei in the bread wheat cultivar Yugoslavia are described. The methodology for detection of interspecies hybrids of common and sessile oak with flourescence in situ hybridization is given. The advantege of the use of total genomic biotin labelled probes for in situ hybridization is that it requires less effort than preparing species specific probes and the ease of interpretation of the results. The technique of fluorescence in situ hybridization is less species than other cytological techniques for higher plants and almost the same methodology can be used for detection of oak hybrids. The extraction of a high quantity of species specific DNA of high quality and probe preparation and labelling are the most important step.
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