Testing for a suitable culture medium for micropropagation of East African highland bananas
1994
Talengera, D. | Magambo, M.J.S. | Rubaihayo, P.R. (Makerere Univ. Kampala (Uganda). Dept. Crop Science)
A study was conducted to find a suitable culture for micropropagation of East African highland bananas (Musa sp.). Modified Eriksson (RE), Gamborg's B-S and Murasinge-Skoog (MS) media were tested with a roasting cultivar Gonja-Horn plantain (AAB), a dessert Bogoya-Gros Michel (AAA) and three East African hihgland cooking (AAA) cultivars Kibuzi, Mbwazirume and Namwezi for in vitro proliferation induction. The media were supplimented with 4.5 mg Benzylaminopurine (BAP) and with or without 0.186 mg Naphthalene acetic acid (NAA). Only ER and MS gave proliferation. AAB cultivar showed precocious rooting in the absence and presence of 0.186 mg NAA on Er and MS media supplimented with 4.5 mg BAP. However, the presence of auxin NAA significantly lowered shoot proliferation with concomitant significant induction of precocious rooting. This indicated a high level of endogenous auxin in these cultivars and therefore, the unnecessity of exogenous auxin for their maximum in vitro proliferation. It was also found that ammoniacal nitrogen content determined the suitability of a culture medium thus making ER an alternative to MS salts
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