Chemotaxonomic characterization of some fast-growing Rhizobia nodulating leguminous trees
1997
Zahran, H.H. (Faculty of Sciences, Beni-Suef (Egypt). Dept. of Botany)
A total of about 50 strains of rhizobia from two leguminous trees (Acacia and Prosopis) were described and compared with 20 reference strains of rhizobia from other tree and herb legumes on the basis of protein, fatty acid and plasmid profiles, and DNA-DNA hybridization. The rhizobia formed thirteen clusters based on protein profile analysis. The rhizobia formed fourteen different clusters based on FAME analysis but the results also conflicted with the phenotypically based methods of analysis. Strains of rhizobia classified in one cluster by any of the above methods of analysis may have shown very different fatty acid profiles. The plasmid profile analysis of the tree rhizobia, on the other hand, was more consistent with the phenotype- and LPS-based numerical analysis. Some strains of the tree rhizobia showed medium or high levels of DNA homology with Rhizobium meliloti. The DNA-DNA hybridization correlated well with protein and fatty acid profiles.
显示更多 [+] 显示较少 [-]