The diagnosis of Fusarium culmorum by polyclonal antibodies - preparation and character of antigens and antibodies
1997
Kratka, J. | Kynerova, B. | Zemanova, A. | Sykorova, S. (Vyzkumny Ustav Rostlinne Vyroby, Prague-Ruzyne (Czech Republic). Odbor Rostlinolekarstvi)
Eighteen monospore Fusarium culmorum (FCu) isolates with different pathogenicity were cultivated on five liquid media. The composition of media influenced morphological and physiological characters of isolates. Antigens were prepared by extraction of proteins from mycelial mats in a special buffer, then purified by centrifugation and ultracentrifugation of supernatant. The composition of media influenced quantity and quality of proteins of antigens. The pathogenicity of FCu isolates was not correlated with protein content of antigens.
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