Some factors affecting anther culture in Linum usitatissimum
1996
Tejklova, E. (AGRITEC, Sumperk-Temenice (Czech Republic))
Influence of genotype, culture conditions of donor plants and culture conditions of isolated anthers on callogenesis in anthers and organogenesis in anther calli as well were studied in Linum usitatissimum. There were differences in anther callogenesis among genotypes. Donor plant culture conditions, especially temperature, influence on callogenesis in anthers. 2,4-D, NAA and picloram induced anther callogenesis in higher frequencis than benzolinon, dicamba, IAA, IBA or TIBA. Callogenesis was higher in dark than in 16-h' day. Cold pretreatment of isolated anthers influenced negatively anther callogenesis. Transfer of calli from induction medium with lower level of some nutrient elements, especially nitrogen, and with higher content of NAA and BAP onto medium with higher level of nutrient elements without auxin and with lower cytokinin level induced bud development in anther calli. Method of production of dihaploid plants in Linum usitatissimum through the anther culture was proposed.
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