An NADH nitrate reductase gene copy appears to have been deleted in barley
1994
Zhou, J. | Kilian, A. | Warner, R.L. | Kleinhofs, A. (Washington State Univ., Pullman (USA). Dept. of Genetics and Cell Biology)
Cultivated barley, Hordeum vulgare L., has a single NADH nitrate reductase (NR) gene while diploid wheat, Triticum monococcum, and cultivated hexaploid wheat, Triticum aestivum L., have two NADH NR genes. To determine whether the NADH NR gene was duplicated since the divergence of Triticum from Hordeum or was deleted from barley, the T. monococcum NADH NR gene heme-hinge regions were sequenced and compared with the barley NADH NR gene sequence. Sequence identity and phylogenetic analyses showed that one of the T. monococcum NADH NR genes is more-closely related to the barley NADH NR gene than to the other T. monococcum NADH NR gene. The heme-hinge region of all 3 NR genes appeared to have evolved at a constant rate. These results suggest that the NADH NR gene duplicated before the divergence of Triticum and Hordeum and that a deletion resulted in the loss of one NADH NR gene from cultivated barley.
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