Transient beta-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens
1998
Rakousky, S. | Ondrej, M. (Akademie Ved, Ceske Budejovice (Czech Republic). Ustav Molekularni Biologie Rostlin) | Kocabek, T. | Vincenciova, R.
Transient expression of the beta-glucuronidase (GUS) gene introduced into Arabidopsis thaliana intact plants by T-DNA after vacuum infiltration of Agrobacterium tumefaciens was followed. The first incidence of GUS activity was found 2-3 d after treatment and a peak of activity one week after treatment in both A. thaliana races, Columbia and C24. GUS activity was sharply increased by cultivation of Arabidopsis plants at elevated temperature (29 deg C) compared to cultivation at 25 deg C. The density of inocula also influenced the GUS activity.
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