Production of sterols by Saccharomyces cerevisiae in fed-batch culture
1998
Vedlichova, Z. | Dubova, R. | Cermak, J. | Patakova, P. | Rychtera, M. | Melzoch, K. (Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Kvasne Chemie a Bioinzenyrstvi)
Experiments were carried out in the fed-batch system and the feeding rate was according to the profile of CO2 in off-gases during cultivation run. Each cultivation was divided into two parts-phase of growth and phase of retarded growth. It was proved that sterols accumulate in cells kept at low growth rate. Also the higher ethanol concentration (50 g/l) in the culture had no stimulating effect for sterol biosynthesis. Nevertheless, ethanol had got positive influence on the ergosterol content in total sterol fraction, that exceeded 95 %. Changes in yeast sterols were observed in the course of storing of biomass at various conditions. The cultivation conditions leading to the different specific biomass growth rates were simulated (using PSI/C simulation software) by means of the mathematical physiological model. Simulationns were verified by cultivations. Model predicted data for profiles CO2 exploited for controlling of feeding were in a good agreement with real courses.
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