New method of preparation of bovine colostral immunoglobulins for parenteral application in calves
1997
Semotan, K. | Kalab, D. (Bioveta, Ivanovice na Hane (Czech Republic))
A new simple method of preparation of bovine colostral immunoglobulins was described using a single step precipitation of skimmed bovine colostrum with dimethyllaurylbenzylammonium bromide (DMLBAB). This quaternary ammonium compound precipitated simultaneously nearly all colostral proteins lacking antibody activity. Bovine colostrum was collected mostly during of the first 24 hours after calving, at the latest however until 48 hours. Isolation of bovine colostral immunoglobulins proceeded as follows: one volume of skimmed colostrum containing 3-6 percent of immunoglobulins was slowly precipitated with the same volume of 2 percent water solution of DMLBAB at pH 7.9-8.1 along with continuous stirring. Average yield of purified colostral immunoglobulins reached 18.8 g/litre of skimmed bovine colostrum
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