Changes in CO2 exchange rate, stomatal conductance, activities of photosystems 1 and 2, and chloroplast polypeptide profile induced by simulated acidic rain
1998
Kumaravelu, G. | Ramanujam, M.P. (K.M. Centre for Post-Graduate Studies, Pondicherry (India). Botany Lab.)
In seedlings of Vigna radiata cultivars ADT-1 and CO-5 exposed to acidic showers (H2SO4:HNO3:HCl,4:2:1, v/v) of different pH (7.0, 5.5, 4.0, and 2.5) for 10 d, net CO2 uptake and stomatal conductance were reduced. The chlorophyll (Chl) a and b contents were reduced but the carotenoid (Car) content increased. In vivo Chl a fluorescence patterns of both the cultivars were altered. No significant change in photosystem (PS) 1 activity was observed except at pH 2.5 where an inhibition was evident. By contrast, PS2 activities declined rapidly with increasing acidity. The room temperature absorption spectra of isolated chloroplasts showed very little changes. SDS-PAGE analysis revealed depletion of 23, 33, and 55 kDa polypeptides. Cultivar CO-5 was more sensitive to acidic rain than cv. ADT-1.
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