Effects of ferulic acid on L-malate oxidation in isolated soybean mitochondria
1998
Sert, M.A. | Ferraresi, M.L.L. | Bernadelli, Y.R. | Kelmer-Bracht, A.M. | Bracht, A. | Ishii-Iwamoto, E.L. (University of Maringa (Brazil))
The effects of ferulic acid on L-malate oxidation in mitochondria isolated from soybean (Glycine max) seedlings were investigated. Oxygen uptake and the products of L-malate oxidation were measured under two conditions: pH 6.8 and 7.8. At acidic pH, the activity of the NAD+-linked malic enzyme was favoured, whereas at alkaline pH a predominance of the L-malate dehydrogenase activity was apparent. Ferulic acid inhibited basal and coupled respiration during L-malate oxidation either at acidic or alkaline pH, reducing also the amounts of pyruvate or oxaloacetate produced. The results suggest that the site of ferulic acid action is situated at some step that precedes the respiratory chain.
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