[Protein fractionation of cassava leaves using sodium dodecil sulphate (SDS) polyacrylamide gel electrophoresis analysis]
1996
Setyani, S. (Universitas Lampung, Bandar Lampung (Indonesia). Fakultas Pertanian)
Cassava leaves can be used as a protein source. However, the type of the protein needs to be identified. One way to identify the species of protein fractions is using electrophoresis techniques. Some factors may influence protein fractionation i.e. the concentration of protein, extraction condition, and protein sources. The purpose of this investigation was to determine the effect of cassava leaves age and antioxidants on the protein fractionation pattern. The experiment included two stages i.e. separations of chloroplastic protein fractions of the old and young cassava leaves and analysis of the protein fraction using SDS polyacrylamide gel electrophoresis. The data were analyzed descriptively. The result showed that the protein fractionation pattern was influenced by addition of antioxidants. Chloroplastic protein of cassava leaves has a range of molecular weight of 40.000 - 50.000 kd, a range which includes albumin
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